Coding
Part:BBa_K5048053:Design
Designed by: Yiheng Liao Group: iGEM24_WHU-China (2024-09-22)
lasR-luxl-dt-plas-ccdB-dt
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 322
Illegal AgeI site found at 519 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1782
Design Notes
We design this part in order to reproduce the result of the literature, except for the double terminators. we discussed with CDG China and they recommended this part because it had high efficiency.
Source
complete synthesis